5K7M

Crystal structure of the catalytic domains of Mettl3/Mettl14 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-18
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.97934
Spacegroup name	P 41 21 2
Unit cell lengths	101.864, 101.864, 117.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.506 - 1.650
R-factor	0.1612
Rwork	0.160
R-free	0.18380
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.932
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.650
Number of reflections	74698
<I/σ(I)>	47.3
Completeness [%]	100.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1 M Tris pH 8.0, 20% PEG3350