5K7C

The native structure of native pistol ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	56.196, 56.196, 89.311
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.700 - 2.730
Rwork	0.200
R-free	0.25000
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.300	2.860
High resolution limit [Å]	2.730	2.730
Rpim	0.037	0.727
Number of reflections	4612
<I/σ(I)>	12.8	0.9
Completeness [%]	99.8
Redundancy	6.2	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Na-cacodylate, pH 6.5, Mg(OAc)2 0.2 M and 32% MPD