5K7B
Beclin 2 CCD homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 95.460, 44.320, 97.957 |
| Unit cell angles | 90.00, 96.77, 90.00 |
Refinement procedure
| Resolution | 40.330 - 2.300 |
| Rwork | 0.221 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.548 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.330 | 2.260 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 17947 | |
| <I/σ(I)> | 7.7 | 2.6 |
| Completeness [%] | 84.6 | 87 |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.1 M Bis-Tris pH 6.5, 0.1 M NaCl and 1.5 M (NH4)2SO4 |
