5K6Z
Sidekick chimera containing sidekick-2 immunoglobulin domains 1-2 and sidekick-1 immunoglobulin domains 3-4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 78.350, 85.730, 146.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.374 - 2.700 |
R-factor | 0.1948 |
Rwork | 0.193 |
R-free | 0.23290 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.715 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.144 | 0.502 |
Number of reflections | 27743 | |
<I/σ(I)> | 7.5 | 3.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6.9 | 295 | 3% (w/v) PEG8000, 40% (v/v) 2-methyl-2,4-pentanediol, 0.1 M sodium cacodylate pH6.9 |