5K6Y

Sidekick-2 immunoglobulin domains 1-4, crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	82.610, 88.290, 106.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.775 - 3.200
R-factor	0.2081
Rwork	0.206
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Sdk2
RMSD bond length	0.003
RMSD bond angle	0.713
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.122	0.487
Number of reflections	12640
<I/σ(I)>	7.1	2.4
Completeness [%]	95.5	96.5
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	5.5	295	24% PEG3350 (w/v), 0.2M sodium chloride, 0.1M Bis-Tris pH5.5