5K6X
Sidekick-2 immunoglobulin domains 1-4, crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.110, 130.010, 78.390 |
| Unit cell angles | 90.00, 100.67, 90.00 |
Refinement procedure
| Resolution | 39.002 - 2.700 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Sdk1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.705 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.099 | 0.487 |
| Number of reflections | 29407 | |
| <I/σ(I)> | 12.2 | 2.8 |
| Completeness [%] | 98.7 | 98.5 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4 | 295 | 1.5M ammonium sulfate, 0.1M sodium citrate pH4 |
