5K6U
Sidekick-1 immunoglobulin domains 1-4, crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.7432 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.072, 49.260, 60.728 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 38.186 - 2.201 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.22840 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.880 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.487 |
| Number of reflections | 22884 | |
| <I/σ(I)> | 12.2 | 2.4 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 295 | 12-16% (w/v) PEG4000, 0.2M ammonium citrate |
