5K6Q
Crystal structure of Arabidopsis thaliana acetohydroxyacid synthase catalytic subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-25 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 178.934, 178.934, 186.086 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.557 - 2.952 |
R-factor | 0.1926 |
Rwork | 0.191 |
R-free | 0.22910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yhy |
RMSD bond length | 0.002 |
RMSD bond angle | 0.603 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.560 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.101 | 0.907 |
Number of reflections | 37387 | |
<I/σ(I)> | 31.3 | 2.1 |
Completeness [%] | 99.7 | 100 |
Redundancy | 15.8 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.8 | 290 | Na/K tartrate, lithium sulfate, CHES, FAD, ThDP, MgCl2 |