5K6O
Structure of a GH3 b-glucosidase from cow rumen metagenome in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.924, 119.251, 157.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.070 - 2.290 |
| R-factor | 0.18358 |
| Rwork | 0.181 |
| R-free | 0.22717 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k6l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.417 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.080 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.090 | 0.528 |
| Number of reflections | 44188 | |
| <I/σ(I)> | 11.4 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2M (NH4)2SO4, 0.1M Bis-Tris pH 5.5, then soaked in 100mM galactose. |
