5K6N
Structure of a GH3 b-glicosidase from cow rumen metagenome in complexed with xylose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.591, 119.324, 157.199 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 95.040 - 2.080 |
R-factor | 0.17576 |
Rwork | 0.174 |
R-free | 0.21254 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k6l |
RMSD bond length | 0.008 |
RMSD bond angle | 1.258 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.040 | 2.140 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.087 | 0.540 |
Number of reflections | 58135 | |
<I/σ(I)> | 10.9 | 3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 2M (NH4)2SO4, 0.1M Bis-Tris pH 5.5, then soaked in 50mM xylose |