5K6M
Structure of a GH3 b-glIcosidase from cow rumen metagenome in complex with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.631, 119.182, 157.417 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 95.020 - 2.170 |
R-factor | 0.17699 |
Rwork | 0.175 |
R-free | 0.21824 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k6l |
RMSD bond length | 0.007 |
RMSD bond angle | 1.218 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.020 | 2.240 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.127 | 0.579 |
Number of reflections | 51199 | |
<I/σ(I)> | 11 | 3.3 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 6.6 | 6.6 |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 25% PEG 3350, 0.2M (NH4)2SO4, 0.1M Bis-Tris pH 5.5, then soaked in 20% glucose. |