5K6L
Structure of a GH3 b-glucosidase from cow rumen metagenome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.215, 119.724, 157.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.310 - 1.830 |
| R-factor | 0.15789 |
| Rwork | 0.157 |
| R-free | 0.17854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x42 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.209 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.310 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.137 | 0.562 |
| Number of reflections | 84644 | |
| <I/σ(I)> | 8.7 | 2.1 |
| Completeness [%] | 98.9 | 87.2 |
| Redundancy | 6.4 | 5.6 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 25% PEG 3350, 0.1M Na malonate pH 7.0, then soaked in 25% glycerol. |
