5K6G
Crystal structure of prefusion-stabilized RSV F single-chain 9-24 DS-Cav1 variant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 168.147, 168.147, 168.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.636 - 2.900 |
| R-factor | 0.2328 |
| Rwork | 0.230 |
| R-free | 0.28710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k6b |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.473 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.640 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.149 | |
| Number of reflections | 18674 | |
| <I/σ(I)> | 13.8 | 1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M NaOAc-Acetic Acid pH 5.5, 1.82 M Li2SO4, 0.1 M MgSO4, 5 % (w/v) PEG 400 |
