5K6F

Crystal structure of prefusion-stabilized RSV F single-chain 9-19 DS-Cav1 variant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 41 3 2
Unit cell lengths	168.934, 168.934, 168.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.150 - 2.590
R-factor	0.2043
Rwork	0.203
R-free	0.23740
RMSD bond length	0.002
RMSD bond angle	0.458
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.854	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	26218
<I/σ(I)>	16.2	1.3
Completeness [%]	100.0
Redundancy	19.9	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Na citrate pH 5.6, 15 % iso-propanol, 17 % PEG 4,000