5K66

Crystal structure of N-terminal amidase with Asn-Glu peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97960
Spacegroup name	I 41 2 2
Unit cell lengths	134.602, 134.602, 119.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.000 - 2.002
R-factor	0.1769
Rwork	0.175
R-free	0.21490
RMSD bond length	0.007
RMSD bond angle	1.037
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.000
High resolution limit [Å]	2.000
Number of reflections	36928
<I/σ(I)>	70.2
Completeness [%]	99.8
Redundancy	15.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M Mg acetate tetrahydrate, 0.2M Na cacodylate pH6.5, 20% (w/v) PEG 8000