5K60

Crystal structure of N-terminal amidase with Gln-Val peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97960
Spacegroup name	I 41 2 2
Unit cell lengths	133.838, 133.838, 119.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.051 - 1.900
R-factor	0.1926
Rwork	0.191
R-free	0.23250
RMSD bond length	0.007
RMSD bond angle	0.999
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.100
High resolution limit [Å]	1.900
Number of reflections	42649
<I/σ(I)>	58.3
Completeness [%]	99.9
Redundancy	16.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M Mg acetate tetrahydrate, 0.2M Na cacodylate pH6.5, 20% (w/v) PEG 8000