5K5Y
Crystal structure of truncated FlgD (monoclinic form) from the human pathogen Helicobacter pylori (strain 26695)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99988 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 77.510, 34.000, 133.340 |
| Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
| Resolution | 62.160 - 2.850 |
| R-factor | 0.22825 |
| Rwork | 0.227 |
| R-free | 0.25561 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zzk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.488 |
| Data reduction software | iMOSFLM (7.2.1.) |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.580 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.586 | |
| Number of reflections | 16477 | |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 94.8 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | PEG 1500, succinic acid, sodium dihydrogen phosphate, glycine |
