5K5W

Crystal structure of limiting CO2-inducible protein LCIB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	43.835, 80.218, 143.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.929 - 2.591
R-factor	0.2687
Rwork	0.269
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b5x
RMSD bond length	0.011
RMSD bond angle	1.355
Data reduction software	SCALA
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.930	2.750
High resolution limit [Å]	2.590	2.590
Number of reflections	16427
<I/σ(I)>	10.45
Completeness [%]	97.3
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	100 mM Bis-Tris, 25% w/v PEG3350