5K5U

Crystal structure of N-terminal amidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-20
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	I 41 2 2
Unit cell lengths	133.680, 133.680, 119.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.892 - 2.700
R-factor	0.2148
Rwork	0.209
R-free	0.26580
RMSD bond length	0.002
RMSD bond angle	0.632
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.900
High resolution limit [Å]	2.700
Number of reflections	14836
<I/σ(I)>	63
Completeness [%]	99.9
Redundancy	16.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M Mg acetate tetrahydrate, 0.2M Na cacodylate pH6.5, 20% (w/v) PEG 8000