Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.650, 56.920, 103.800 |
| Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
| Resolution | 48.018 - 2.649 |
| R-factor | 0.2615 |
| Rwork | 0.260 |
| R-free | 0.27950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rs8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.753 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.018 |
| High resolution limit [Å] | 2.649 |
| Number of reflections | 22831 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 92.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 10% PEG 3000, 0.1 M sodium phosphate/citrate pH 6.2 |
