5K5P
Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K L103K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-05-06 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.120, 61.640, 80.399 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.920 - 1.830 |
| R-factor | 0.19096 |
| Rwork | 0.188 |
| R-free | 0.23744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.813 |
| Data reduction software | XPREP |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.057 | 0.221 |
| Number of reflections | 13844 | |
| <I/σ(I)> | 60.29 | 13.35 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.4 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
