5K5O
Structure of AspA-26mer DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 96.627, 96.627, 187.093 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.005 - 3.200 |
| R-factor | 0.2602 |
| Rwork | 0.257 |
| R-free | 0.28940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rs8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.473 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.005 | 3.320 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.145 | 0.550 |
| Number of reflections | 14191 | |
| <I/σ(I)> | 6.5 | 1.1 |
| Completeness [%] | 84.6 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1 M citrate, 0.1 M cacodylate |
