5K5I
Homo sapiens CCCTC-binding factor (CTCF) ZnF5-8 and DNA complex structure in space group P65
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-09 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.27046 |
| Spacegroup name | P 65 |
| Unit cell lengths | 45.075, 45.075, 260.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.247 - 2.190 |
| R-factor | 0.2198 |
| Rwork | 0.218 |
| R-free | 0.24470 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.448 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((dev_2400: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.247 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.105 | 0.674 |
| Number of reflections | 30205 | |
| <I/σ(I)> | 19.29 | 2.333 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 14.2 | 6.5 |
| CC(1/2) | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 20% (w/v) polyethylene glycol 3350, 0.2 M ammonium sulfate, 0.1 M sodium cacodylate pH 6.5 |
