5K5D

Structure of the C2221 form of Pnob8-like ParB-N domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	71.030, 98.700, 307.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.107 - 2.450
R-factor	0.2243
Rwork	0.222
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k5a
RMSD bond length	0.003
RMSD bond angle	0.629
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.107	2.540
High resolution limit [Å]	2.450	2.450
Number of reflections	38446
<I/σ(I)>	10.9	1.9
Completeness [%]	95.6	83.8
Redundancy	4.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350,