5K55
Human muscle fructose-1,6-bisphosphatase E69Q mutant in active R-state in complex with fructose-6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-22 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 72.317, 72.317, 231.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.029 - 1.977 |
| R-factor | 0.1876 |
| Rwork | 0.185 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5et5 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.621 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.030 | 2.100 |
| High resolution limit [Å] | 1.977 | 1.977 |
| Rmerge | 0.076 | 0.761 |
| Number of reflections | 22004 | |
| <I/σ(I)> | 22.79 | 3.15 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 9.56 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | 10mM magnesium chloride, 2M sodium chloride, 10% PEG6000, 10mM Tris |
