5K4F
CAL PDZ mutant C319A with a peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.730, 48.276, 51.435 |
| Unit cell angles | 90.00, 101.76, 90.00 |
Refinement procedure
| Resolution | 18.791 - 1.360 |
| R-factor | 0.1552 |
| Rwork | 0.154 |
| R-free | 0.18330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e34 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.879 |
| Data scaling software | XDS (20151015) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.791 | 1.460 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.047 | 0.540 |
| Number of reflections | 33527 | |
| <I/σ(I)> | 17.23 | 3.09 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 4.26 | 4.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 31% PEG3350, 125mM NaCl, 0.1M Tris7.5 |
