5K4D
Structure of eukaryotic translation initiation factor 3 subunit D (eIF3d) cap binding domain from Nasonia vitripennis, Crystal form 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.975, 144.325, 55.302 |
| Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
| Resolution | 49.132 - 2.000 |
| R-factor | 0.1785 |
| Rwork | 0.177 |
| R-free | 0.20800 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.638 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.132 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.523 |
| Number of reflections | 49757 | |
| <I/σ(I)> | 6.5 | 1.4 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 200 mM NaCl, 100 mM Tris 8.5, 25% PEG-3350 |
