5K4C
Structure of eukaryotic translation initiation factor 3 subunit D (eIF3d) cap binding domain from Nasonia vitripennis, Crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-06-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11586 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.011, 61.844, 138.311 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.099 - 1.698 |
| R-factor | 0.1634 |
| Rwork | 0.162 |
| R-free | 0.19630 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.359 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 1.730 |
| High resolution limit [Å] | 1.698 | 1.698 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 47123 | |
| <I/σ(I)> | 9.1 | 1.6 |
| Completeness [%] | 99.4 | 89.4 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.6-1.8 M Ammonium Citrate pH 7.0 |
