5K4B
Structure of eukaryotic translation initiation factor 3 subunit D (eIF3d) cap binding domain from Nasonia vitripennis, Crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-06-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11586 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.832, 62.909, 192.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.028 - 1.399 |
| R-factor | 0.1748 |
| Rwork | 0.174 |
| R-free | 0.19310 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.167 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.060 | 1.420 |
| High resolution limit [Å] | 1.399 | 1.399 |
| Rmerge | 0.026 | 0.453 |
| Number of reflections | 148455 | |
| <I/σ(I)> | 12.9 | 1.5 |
| Completeness [%] | 100.0 | 99.2 |
| Redundancy | 15.8 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 200 mM (NH4)2SO4, 100 mM Bis-Tris 6.5, 23-27% PEG-3350 |
