5K3V
apo-PDX1.3 (Arabidopsis)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97858 |
| Spacegroup name | H 3 |
| Unit cell lengths | 178.220, 178.220, 116.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 92.949 - 1.900 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nv2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.723 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 92.949 | 46.475 | 1.540 |
| High resolution limit [Å] | 1.461 | 4.620 | 1.461 |
| Rmerge | 0.040 | 4.691 | |
| Number of reflections | 236195 | ||
| <I/σ(I)> | 4.4 | 13 | 0.2 |
| Completeness [%] | 98.8 | 98.6 | 97.8 |
| Redundancy | 4.3 | 4.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.05 M MES.OH pH 6.5, 0.7 M ammonium sulfate, 5% 1,4-dioxane, 0.01 M Tris.HCl pH 7.0, 0.1 M potassium chloride, and 0.005 M dithiothreitol |
