5K3V
apo-PDX1.3 (Arabidopsis)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97858 |
Spacegroup name | H 3 |
Unit cell lengths | 178.220, 178.220, 116.430 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 92.949 - 1.900 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.20330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nv2 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.723 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 92.949 | 46.475 | 1.540 |
High resolution limit [Å] | 1.461 | 4.620 | 1.461 |
Rmerge | 0.040 | 4.691 | |
Number of reflections | 236195 | ||
<I/σ(I)> | 4.4 | 13 | 0.2 |
Completeness [%] | 98.8 | 98.6 | 97.8 |
Redundancy | 4.3 | 4.7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.05 M MES.OH pH 6.5, 0.7 M ammonium sulfate, 5% 1,4-dioxane, 0.01 M Tris.HCl pH 7.0, 0.1 M potassium chloride, and 0.005 M dithiothreitol |