5K3Q

Rhesus macaques Trim5alpha Bbox2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-25
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	30.751, 54.556, 54.479
Unit cell angles	90.00, 100.03, 90.00

Refinement procedure

Resolution	38.250 - 1.800
R-factor	0.1801
Rwork	0.177
R-free	0.22910
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.159
Data reduction software	XDS
Data scaling software	Aimless (0.5.15)
Phasing software	SHELX (2006/3)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.250	38.250	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.089	0.052	0.907
Number of reflections	16550
<I/σ(I)>	17.6
Completeness [%]	100.0	99.2	100
Redundancy	7.6	6.6	7.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	24% (w/v) PE3350, 0.4 M LiNO3