5K3O
Wolinella succinogenes L-asparaginase P121 and L-Aspartic acid
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.755, 71.459, 141.502 |
| Unit cell angles | 90.00, 118.07, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.696 |
| R-factor | 0.1768 |
| Rwork | 0.175 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wsa |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.776 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.696 | 1.696 |
| Number of reflections | 139440 | |
| <I/σ(I)> | 16.37 | |
| Completeness [%] | 98.8 | 97.2 |
| Redundancy | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | PEG2000, HEPES 7.5 |
