5K3G
Crystals structure of Acyl-CoA oxidase-1 in Caenorhabditis elegans, Apo form-I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.433, 140.547, 131.335 |
| Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
| Resolution | 48.851 - 2.859 |
| R-factor | 0.2169 |
| Rwork | 0.216 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1is2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.795 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.851 | 2.960 |
| High resolution limit [Å] | 2.859 | 2.859 |
| Rmerge | 0.140 | 0.560 |
| Number of reflections | 67386 | |
| <I/σ(I)> | 12.2 | 0.61 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.4 M NaCl, 0.1 M HEPES pH 7.4, 18% w/v PEG 8000 and 8% v/v glycerol |
