5K39
THE TYPE II COHESIN DOCKERIN COMPLEX FROM CLOSTRIDIUM THERMOCELLUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9735 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.670, 78.630, 35.800 |
| Unit cell angles | 90.00, 95.87, 90.00 |
Refinement procedure
| Resolution | 18.830 - 1.980 |
| R-factor | 0.19331 |
| Rwork | 0.187 |
| R-free | 0.24739 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2MB3 AND 2B59 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.154 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.310 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.090 | 0.170 |
| Number of reflections | 21909 | |
| <I/σ(I)> | 13.1 | 5.5 |
| Completeness [%] | 97.8 | 98.2 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% (m/v) polyethyleneglycol (PEG) 3350, 4% tacsimate, pH 5.0 |
