5K35

Structure of the Legionella effector, AnkB, in complex with human Skp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-21
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9770
Spacegroup name	P 21 21 21
Unit cell lengths	53.576, 57.039, 150.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.850
R-factor	0.22203
Rwork	0.219
R-free	0.27473
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.253
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge		0.622
Number of reflections	10735
<I/σ(I)>	48.5	6.42
Completeness [%]	100.0	100
Redundancy	14.2	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M trimethylamine N-oxide, 0.1 M Tris pH 8.5, 20% (w/v) PEG 2000 MME