5K2S

Lysozyme with nano particles

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	77.388, 77.388, 37.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.347 - 1.550
R-factor	0.157
Rwork	0.155
R-free	0.18530
RMSD bond length	0.017
RMSD bond angle	1.754
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.580
High resolution limit [Å]	1.550	4.200	1.550
Rmerge	0.071	0.026	0.397
Rmeas	0.074	0.028	0.413
Rpim	0.021	0.008	0.115
Total number of observations	212688
Number of reflections	17200
<I/σ(I)>	7.5
Completeness [%]	100.0	100	100
Redundancy	12.4	11.2	12.8
CC(1/2)		0.999	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Sodium Chloride, 0.1M Sodium Citrate-Citric Acid pH5.5, 1.0M Ammonium Phosphate Dibasic