5K2R
Crystal structure of lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.485, 77.485, 38.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.371 - 1.600 |
R-factor | 0.1766 |
Rwork | 0.174 |
R-free | 0.21680 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.830 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.630 |
High resolution limit [Å] | 1.600 | 4.330 | 1.600 |
Rmerge | 0.095 | 0.064 | 0.642 |
Rmeas | 0.099 | 0.067 | 0.673 |
Rpim | 0.028 | 0.019 | 0.197 |
Total number of observations | 194685 | ||
Number of reflections | 15845 | ||
<I/σ(I)> | 5.5 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 12.3 | 11.1 | 11.4 |
CC(1/2) | 0.997 | 0.922 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl pH 8.5, 1.5M Ammonium Sulfate |