5K2P
Crystal structure of lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.956, 76.956, 37.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.478 - 1.240 |
| R-factor | 0.1355 |
| Rwork | 0.134 |
| R-free | 0.17280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.761 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.260 |
| High resolution limit [Å] | 1.240 | 3.370 | 1.240 |
| Rmerge | 0.060 | 0.032 | 0.673 |
| Rmeas | 0.063 | 0.034 | 0.706 |
| Rpim | 0.019 | 0.010 | 0.213 |
| Total number of observations | 361555 | ||
| Number of reflections | 32317 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 99.9 | 99.6 | 99.8 |
| Redundancy | 11.2 | 11.1 | 10.8 |
| CC(1/2) | 0.999 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl pH8.5, 1.3M Ammonium Tartrate Dibasic |
