5K2L

Crystal structure of LysM domain from Volvox carteri chitinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2014-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	29.130, 29.130, 94.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.830 - 1.200
R-factor	0.14073
Rwork	0.139
R-free	0.17959
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pxv
RMSD bond length	0.026
RMSD bond angle	1.999
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	13481
<I/σ(I)>	73
Completeness [%]	99.5
Redundancy	23.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium citrate tribasic dihydrate, 20 % PEG 3350