5K2H
Structure of GNNQQNY from yeast prion Sup35 in space group P212121 determined by MicroED
Experimental procedure
Source type | ELECTRON MICROSCOPE |
Source details | TECNAI F20 TEM |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-02-03 |
Detector | TVIPS F416 CMOS CAMERA |
Wavelength(s) | 0.0251 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 23.160, 4.930, 40.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.110 - 1.050 |
R-factor | 0.1781 |
Rwork | 0.177 |
R-free | 0.18560 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.592 |
Data scaling software | XSCALE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.110 | 1.190 | |
High resolution limit [Å] | 1.050 | 1.710 | 1.050 |
Rmerge | 0.189 | 0.116 | 0.438 |
Rmeas | 0.218 | 0.137 | 0.497 |
Total number of observations | 6795 | ||
Number of reflections | 1836 | 427 | 572 |
<I/σ(I)> | 4.18 | 7.47 | 2.26 |
Completeness [%] | 72.7 | 67.5 | 75.1 |
CC(1/2) | 0.992 | 0.993 | 0.813 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 7 | 273 | water |