5K2H

Structure of GNNQQNY from yeast prion Sup35 in space group P212121 determined by MicroED

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	TECNAI F20 TEM
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-02-03
Detector	TVIPS F416 CMOS CAMERA
Wavelength(s)	0.0251
Spacegroup name	P 21 21 21
Unit cell lengths	23.160, 4.930, 40.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.110 - 1.050
R-factor	0.1781
Rwork	0.177
R-free	0.18560
RMSD bond length	0.017
RMSD bond angle	1.592
Data scaling software	XSCALE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.110		1.190
High resolution limit [Å]	1.050	1.710	1.050
Rmerge	0.189	0.116	0.438
Rmeas	0.218	0.137	0.497
Total number of observations	6795
Number of reflections	1836	427	572
<I/σ(I)>	4.18	7.47	2.26
Completeness [%]	72.7	67.5	75.1
CC(1/2)	0.992	0.993	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH	7	273	water