5K2G
Structure of GNNQQNY from yeast prion Sup35 in space group P21 determined by MicroED
Experimental procedure
| Source type | ELECTRON MICROSCOPE |
| Source details | TECNAI F20 TEM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-03 |
| Detector | TVIPS F416 CMOS CAMERA |
| Wavelength(s) | 0.0251 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 22.870, 4.930, 24.180 |
| Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
| Resolution | 23.030 - 1.100 |
| R-factor | 0.1902 |
| Rwork | 0.187 |
| R-free | 0.22420 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.337 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 7 | 273 | water |
