5K2E
Structure of NNQQNY from yeast prion Sup35 with zinc acetate determined by MicroED
Experimental procedure
Source type | ELECTRON MICROSCOPE |
Source details | TECNAI F20 TEM |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-02-03 |
Detector | TVIPS F416 CMOS CAMERA |
Wavelength(s) | 0.0251 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 21.480, 4.950, 23.870 |
Unit cell angles | 90.00, 103.95, 90.00 |
Refinement procedure
Resolution | 23.170 - 1.000 |
R-factor | 0.1564 |
Rwork | 0.152 |
R-free | 0.19430 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.525 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 1.080 |
High resolution limit [Å] | 1.000 | 1.710 | 1.000 |
Rmerge | 0.211 | 0.154 | 0.362 |
Total number of observations | 13199 | ||
Number of reflections | 2712 | ||
<I/σ(I)> | 4.1 | ||
Completeness [%] | 85.8 | 85.8 | 79.9 |
Redundancy | 4.9 | 5.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 273 | 1.0 M sodium acetate, 0.1 M HEPES, pH 7.0, 0.01 M zinc sulfate |