5K23
Crystal structure of the complex between human phosphatase PRL-2 in the oxidized state with the Bateman domain of human magnesium transporter CNNM3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9759 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 51.156, 124.490, 159.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.020 - 2.960 |
| R-factor | 0.20786 |
| Rwork | 0.205 |
| R-free | 0.26365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zck |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.875 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.040 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Rmerge | 0.436 | |
| Number of reflections | 10491 | |
| <I/σ(I)> | 33.2 | 3.3 |
| Completeness [%] | 99.5 | 94.06 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.54M sodium citrate, 0.1M sodium acetate, 10mM yttrium chloride |
