5K1F
Crystal structure of a class C beta lactamase/compound2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.28270 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.698, 59.377, 113.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.532 - 1.940 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zkj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.086 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.940 |
| Number of reflections | 25473 |
| <I/σ(I)> | 57.54 |
| Completeness [%] | 99.4 |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 295 | Cadmium chloride, Na-Cacodylate, PEG 400 |
