5K17

Crystal structure of CREN7-DSDNA (GTGATCGC) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97947
Spacegroup name	C 2 2 21
Unit cell lengths	77.694, 77.676, 104.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.000 - 2.100
R-factor	0.19636
Rwork	0.196
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lwh
RMSD bond length	0.009
RMSD bond angle	1.212
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	18763
<I/σ(I)>	3.82
Completeness [%]	99.9
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	PEG1500