5K08
RecA mini intein-Zeise's salt complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-26 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.07136 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.687, 63.930, 37.307 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.222 - 1.401 |
R-factor | 0.1822 |
Rwork | 0.180 |
R-free | 0.20990 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5i0a |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.420 |
High resolution limit [Å] | 1.400 | 3.800 | 1.400 |
Rmerge | 0.088 | 0.071 | 0.913 |
Rmeas | 0.091 | ||
Rpim | 0.025 | ||
Total number of observations | 373588 | ||
Number of reflections | 27687 | ||
<I/σ(I)> | 10.8 | ||
Completeness [%] | 99.4 | 98.4 | 96 |
Redundancy | 13.5 | 12.3 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 54%-59% (w/v) ammonium sulfate, 0.1 M Tris, pH 8.5, 2 mM Zeise's salt, 2 mM TCEP |