5JZO
Structure of wild type amidase at high temperature at 2.5 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2013-12-07 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.375, 80.069, 133.133 |
Unit cell angles | 90.00, 95.35, 90.00 |
Refinement procedure
Resolution | 29.668 - 2.500 |
R-factor | 0.1798 |
Rwork | 0.177 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n57 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.315 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.668 | 2.670 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.057 | |
Number of reflections | 49277 | |
<I/σ(I)> | 1.39 | |
Completeness [%] | 92.3 | |
Redundancy | 1.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 5% PEG 6000, 0.1M sodium citrate, 8% MPD against a reservoir solution of 15% PEG 6000, 0.1M tris-HCl, pH-8.5, 5% MPD. |