5JZM
Structure of wild type Amidase from Vibrio cholerae 0395 at low temparature at 1.8 Angstroms resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-11-30 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.920, 79.720, 133.580 |
Unit cell angles | 90.00, 95.04, 90.00 |
Refinement procedure
Resolution | 36.313 - 1.870 |
R-factor | 0.1589 |
Rwork | 0.158 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n57 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.352 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.313 | 1.960 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.086 | |
Number of reflections | 118593 | |
<I/σ(I)> | 1.34 | |
Completeness [%] | 93.0 | |
Redundancy | 1.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 5% PEG 6000, 0.1M Na-citrate, 8% MPD |