5JZE

Erve virus viral OTU domain protease in complex with mouse ISG15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 43
Unit cell lengths	65.992, 65.992, 121.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.990 - 2.470
R-factor	0.1767
Rwork	0.174
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hxd
RMSD bond length	0.014
RMSD bond angle	1.718
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.990	50.000	2.510
High resolution limit [Å]	2.470	6.700	2.470
Rmerge	0.119	0.035	0.480
Number of reflections	18742
<I/σ(I)>	5.4
Completeness [%]	100.0	99.8	100
Redundancy	4.2	4.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	6% PEG 6000 and 0.1 M citric acid supplemented 0.2% of 3.0 M NTSB-195