5JYM
Human P-cadherin EC12 with scFv TSP11 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 58.700, 83.430, 199.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.910 - 2.450 |
R-factor | 0.22569 |
Rwork | 0.223 |
R-free | 0.27037 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qok |
RMSD bond length | 0.011 |
RMSD bond angle | 1.468 |
Data reduction software | MOSFLM (7.0.9) |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.910 | 2.580 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.084 | 0.540 |
Number of reflections | 36872 | |
<I/σ(I)> | 14.5 | 2.8 |
Completeness [%] | 99.6 | 97.6 |
Redundancy | 6.7 | 6.7 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 150 mM CaCl2, 16% PEG 3350 |